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MBC in Press, published online ahead of print November 7, 2007
Mol. Biol. Cell 10.1091/mbc.E07-05-0519

A more recent version of this article appeared on January 1, 2008
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Submitted on June 1, 2007
Revised on September 10, 2007
Accepted on October 29, 2007

Large-Scale Analysis of Yeast Filamentous Growth by Systematic Gene Disruption and Overexpression

Rui Jin, Craig J. Dobry, Phillip J. McCown, and Anuj Kumar

Department of Molecular, Cellular, and Developmental Biology and Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2216

Monitoring Editor: Charles Boone

Under certain conditions of nutrient stress, the budding yeast S. cerevisiae initiates a striking developmental transition to a filamentous form of growth, resembling developmental transitions required for virulence in closely related pathogenic fungi. In yeast, filamentous growth involves known MAPK and PKA signaling modules, but the full scope of this extensive filamentous response has not been delineated. Accordingly, we have undertaken the first systematic gene disruption and overexpression analysis of yeast filamentous growth. Standard laboratory strains of yeast are nonfilamentous; thus, we constructed a unique set of reagents in the filamentous {Sigma}1278b strain, encompassing 3627 integrated transposon insertion alleles and 2043 overexpression constructs. Collectively, we analyzed 4528 yeast genes with these reagents and identified 487 genes conferring mutant filamentous phenotypes upon transposon insertion and/or gene overexpression. Using a fluorescent protein reporter integrated at the MUC1 locus, we further assayed each filamentous growth mutant for aberrant protein levels of the key flocculence factor Muc1p. Our results indicate a variety of genes and pathways affecting filamentous growth. In total, this filamentous growth gene set represents a wealth of yeast biology, highlighting 84 genes of uncharacterized function and an underappreciated role for the mitochondrial retrograde signaling pathway as an inhibitor of filamentous growth.

Address correspondence to: Anuj Kumar (anujk{at}umich.edu)




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N. Bharucha, J. Ma, C. J. Dobry, S. K. Lawson, Z. Yang, and A. Kumar
Analysis of the Yeast Kinome Reveals a Network of Regulated Protein Localization during Filamentous Growth
Mol. Biol. Cell, July 1, 2008; 19(7): 2708 - 2717.
[Abstract] [Full Text] [PDF]


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