Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print February 13, 2008
Mol. Biol. Cell 10.1091/mbc.E07-07-0652

A more recent version of this article appeared on April 1, 2008
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Materials
Right arrow All Versions of this Article:
E07-07-0652v1
19/4/1772    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sherwood, V.
Right arrow Articles by Chalmers, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sherwood, V.
Right arrow Articles by Chalmers, A. D.

Submitted on July 10, 2007
Revised on January 9, 2008
Accepted on February 4, 2008

RASSF7 is a Member of a New Family of RAS Association Domain-Containing Proteins and is Required for Completing Mitosis

Victoria Sherwood, Ria Manbodh, Carol Sheppard, and Andrew D. Chalmers

Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom

Monitoring Editor: Marianne Bronner-Fraser

Mitosis is a fundamental feature of all cellular organisms. It must be tightly regulated to allow normal tissue growth and to prevent cancer formation. Here, we identify a new protein that is required for mitosis. We show that the Ras association (RA) domain containing protein, RASSF7, is part of an evolutionarily conserved group of four proteins. These are RASSF7, RASSF8 and two new RASSF proteins P-CIP1/RASSF9 and RASSF10. We call this group the N-terminal RASSF family. We analyzed the function of Xenopus RASSF7. RASSF7 was found to be expressed in several embryonic tissues including the skin, eyes and neural tube. Knocking down its function led to cells failing to form a mitotic spindle and arresting in mitosis. This caused nuclear breakdown, apoptosis and a striking loss of tissue architecture in the neural tube. Consistent with a role in spindle formation, RASSF7 protein was found to localize to the centrosome. This localization occurred in a microtubule-dependent manner, demonstrating that there is a mutually dependant relationship between RASSF7 localization and spindle formation. Thus RASSF7, the first member of the N-terminal RASSF family to be functionally analyzed, is a centrosome-associated protein required to form a spindle and complete mitosis in the neural tube.

Address correspondence to: Andrew D. Chalmers (ac270{at}bath.ac.uk)




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. Rajagopal, K. L. Stone, V. P. Francone, R. E. Mains, and B. A. Eipper
Secretory Granule to the Nucleus: ROLE OF A MULTIPLY PHOSPHORYLATED INTRINSICALLY UNSTRUCTURED DOMAIN
J. Biol. Chem., September 18, 2009; 284(38): 25723 - 25734.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. J. Foley, H. Freedman, S. L. Choo, C. Onyskiw, N. Y. Fu, V. C. Yu, J. Tuszynski, J. C. Pratt, and S. Baksh
Dynamics of RASSF1A/MOAP-1 Association with Death Receptors
Mol. Cell. Biol., July 15, 2008; 28(14): 4520 - 4535.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2008 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.