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MBC in Press, published online ahead of print September 19, 2007
Mol. Biol. Cell 10.1091/mbc.E07-07-0657

A more recent version of this article appeared on December 1, 2007
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Submitted on July 11, 2007
Revised on September 5, 2007
Accepted on September 10, 2007

Role of Hsp104 in the Propagation and Inheritance of the [Het-s] Prion

Laurent Malato, Suzana Dos Reis, Laura Benkemoun, Raimon Sabaté, and Sven J. Saupe

Laboratoire de Génétique Moléculaire des Champignons, Institut de Biochimie et de Génétique Cellulaires, Unité Mixte de Recherche 5095 Centre National de la Recherche Scientifique/Université de Bordeaux 2, 33077 Bordeaux Cedex, France

Monitoring Editor: Jonathan Weissman

The chaperones of the ClpB/HSP100 family play a central role in thermotolerance in bacteria, plants and fungi by ensuring solubilization of heat-induced protein aggregates. In addition in yeast, Hsp104 was found to be required for prion propagation. Herein, we analyze the role of Podospora anserina Hsp104 (PaHsp104) in the formation and propagation of the [Het-s] prion. We show that {Delta}PaHSP104 strains propagate [Het-s], making [Het-s] the first native fungal prion to be propagated in the absence of Hsp104. Nevertheless, we found that [Het-s]-propagon numbers, propagation rate and spontaneous emergence are reduced in a {Delta}PaHSP104 background. In addition, inactivation of PaHsp104 leads to severe meiotic instability of [Het-s] and abolishes its meiotic drive activity. Finally, we show that {Delta}PaHSP104 strains are less susceptible than wild type to infection by exogenous recombinant HET-s(218–289) prion amyloids. Like [URE3] and [PIN+] in yeast but unlike [PSI+], [Het-s] is not cured by constitutive PaHsp104 overexpression. The observed effects of PaHsp104 inactivation are consistent with the described role of Hsp104 in prion aggregate shearing in yeast. However, Hsp104-dependency appears less stringent in P. anserina than in yeast; presumably because in Podospora prion propagation occurs in a syncitium.

Address correspondence to: Sven J. Saupe (sven.saupe{at}ibgc.u-bordeaux2.fr)






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