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MBC in Press, published online ahead of print January 23, 2008
Mol. Biol. Cell 10.1091/mbc.E07-07-0659

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Submitted on July 12, 2007
Revised on January 9, 2008
Accepted on January 15, 2008

Global Analysis of Yeast Endosomal Transport Identifies the Vps55/68 Sorting Complex

Cayetana Schluter,* Karen K. Y. Lam,* Jochen Brumm,{dagger} Bella W. Wu,* Matthew Saunders,{ddagger} Tom H. Stevens,{ddagger} Jennifer Bryan,{dagger} and Elizabeth Conibear*

*Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada; {dagger}Department of Statistics and Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T1Z2, Canada; {ddagger}Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229

Monitoring Editor: Sandra Lemmon

Endosomal transport is critical for cellular processes ranging from receptor down-regulation and retroviral budding to the immune response. A full understanding of endosome sorting requires a comprehensive picture of the multiprotein complexes that orchestrate vesicle formation and fusion. Here, we use unsupervised, large-scale phenotypic analysis and a novel computational approach for the global identification of endosomal transport factors. This technique effectively identifies components of known and novel protein assemblies. We report the characterization of a previously undescribed endosome sorting complex that contains two well-conserved proteins with 4 predicted membrane-spanning domains. Vps55p and Vps68p form a complex that acts with or downstream of ESCRT function to regulate endosomal trafficking. Loss of Vps68p disrupts recycling to the TGN as well as onward trafficking to the vacuole without preventing the formation of lumenal vesicles within the MVB. Our results suggest the Vps55/68 complex mediates a novel, conserved step in the endosomal maturation process.

Address correspondence to: Elizabeth Conibear (jenny{at}stat.ubc.ca)




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H. E. Burston, L. Maldonado-Baez, M. Davey, B. Montpetit, C. Schluter, B. Wendland, and E. Conibear
Regulators of yeast endocytosis identified by systematic quantitative analysis
J. Cell Biol., June 15, 2009; 185(6): 1097 - 1110.
[Abstract] [Full Text] [PDF]


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