Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print April 2, 2008
Mol. Biol. Cell 10.1091/mbc.E07-10-0998

A more recent version of this article appeared on June 1, 2008
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E07-10-0998v1
19/6/2579    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duran, J. M.
Right arrow Articles by Malhotra, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duran, J. M.
Right arrow Articles by Malhotra, V.

Submitted on October 3, 2007
Revised on March 17, 2008
Accepted on March 20, 2008

The Role of GRASP55 in Golgi Fragmentation and Entry of Cells into Mitosis

Juan Manuel Duran,*{dagger} Matt Kinseth,{dagger}{ddagger} Carine Bossard,{ddagger} David W. Rose,{sect} Roman Polishchuk,|| Christine C. Wu,¶ John Yates,# Timo Zimmerman,* and Vivek Malhotra*

*Cell and Development Program, Centro de Regulacion Genomica (CRG), 08003 Barcelona, Spain; {ddagger}Department of Cell and Developmental Biology, {sect}Department of Medicine, Cancer Center, University of California, San Diego, LA Jolla, CA 92093; ||Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030, Santa Maria Imbaro (Chieti), Italy; Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045; #Department of Cell Biology, Scripps Research Institute, LA Jolla, CA 92037

Monitoring Editor: Benjamin Glick

GRASP55 is a Golgi associated protein but its function at the Golgi remains unclear. Addition of full length GRASP55, GRASP55-specific peptides or an anti-GRASP55 antibody inhibited Golgi fragmentation by mitotic extracts in vitro, and entry of cells into mitosis. Phospho-peptide mapping of full length GRASP55 revealed that threonine 225 and 249 were mitotically phosphorylated. Wild type peptides containing T225 and T249 inhibited Golgi fragmentation and entry of cells into mitosis. Mutant peptides containing T225E and T249E, on the other hand, did not affect Golgi fragmentation and entry into mitosis. These findings reveal a role of GRASP55 in events leading to Golgi fragmentation and the subsequent entry of cell into mitosis. Surprisingly, however, under our experimental conditions, greater than 85% knockdown of GRASP55did not affect the overall organization of Golgi organization in terms of cisternal stacking and lateral connections between stacks. Based on our findings we suggest that phosphorylation of GRASP55 at T225/T249 releases a bound component, which is phosphorylated and necessary for Golgi fragmentation. Thus GRASP55 has no role in the organization of Golgi membranes per se but controls their fragmentation by regulating the release of a partner, which requires a G2 specific phosphorylation at T225/T249.

{dagger}These authors contributed equally to this work.

Address correspondence to: Vivek Malhotra (vivek.malhotra{at}crg.es)




This article has been cited by other articles:


Home page
 JCBHome page
Y. Xiang and Y. Wang
GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking
J. Cell Biol., January 25, 2010; 188(2): 237 - 251.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. D'Angelo, L. Prencipe, L. Iodice, G. Beznoussenko, M. Savarese, P. Marra, G. Di Tullio, G. Martire, M. A. De Matteis, and S. Bonatti
GRASP65 and GRASP55 Sequentially Promote the Transport of C-terminal Valine-bearing Cargos to and through the Golgi Complex
J. Biol. Chem., December 11, 2009; 284(50): 34849 - 34860.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2008 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.