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MBC in Press, published online ahead of print July 15, 2009
Mol. Biol. Cell 10.1091/mbc.E09-04-0330

A more recent version of this article appeared on September 1, 2009
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Submitted on April 27, 2009
Revised on June 25, 2009
Accepted on July 2, 2009

Pericentromeric Sister Chromatid Cohesion Promotes Kinetochore Biorientation

Tessie M. Ng,*{dagger} William G. Waples,{ddagger} Brigitte D. Lavoie,{ddagger} and Sue Biggins*

*Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109; {dagger}Molecular and Cellular Biology Program, University of Washington and Fred Hutchinson Cancer Research Center, Seattle, WA; {ddagger}Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada M5S 1A8

Monitoring Editor: Kerry S. Bloom

Accurate chromosome segregation depends on sister kinetochores making bioriented attachments to microtubules from opposite poles. An essential regulator of biorientation is the Ipl1/Aurora B protein kinase that destabilizes improper microtubule-kinetochore attachments. To identify additional biorientation pathways, we performed a systematic genetic analysis between the ipl1–321 allele and all nonessential budding yeast genes. One of the mutants, mcm21 {Delta}, precociously separates pericentromeres and this is associated with a defect in the binding of the Scc2 cohesin-loading factor at the centromere. Strikingly, Mcm21 becomes essential for biorientation when Ipl1 function is reduced, and this appears to be related to its role in pericentromeric cohesion. When pericentromeres are artificially tethered, Mcm21 is no longer needed for biorientation despite decreased Ipl1 activity. Taken together, these data reveal a specific role for pericentromeric linkage in ensuring kinetochore biorientation.

Address correspondence to: Sue Biggins (sbiggins{at}fhcrc.org)




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B. Akiyoshi, C. R. Nelson, J. A. Ranish, and S. Biggins
Analysis of Ipl1-Mediated Phosphorylation of the Ndc80 Kinetochore Protein in Saccharomyces cerevisiae
Genetics, December 1, 2009; 183(4): 1591 - 1595.
[Abstract] [Full Text] [PDF]


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