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Vol. 14, Issue 12, 4920-4930, December 2003

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The Adhesin Hwp1 and the First Daughter Cell Localize to the a/a Portion of the Conjugation Bridge during Candida albicans Mating

Karla J. Daniels ^*, Shawn R. Lockhart ^*, Janet F. Staab , Paula Sundstrom , and David R. Soll ^* 

^* Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242-1324; Department of Medical Microbiology and Immunology, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio 43210

Submitted April 29, 2003; Revised July 24, 2003; Accepted August 13, 2003
Monitoring Editor: Randy Schekman

The cell wall protein Hwp1 was originally demonstrated to be expressed exclusively in hyphae of Candida albicans and cross-linked to human epithelium by mammalian transglutaminase. Hwp1 is expressed on the walls of hyphae formed by a/, a/a, and / cells. Hence, it is expressed on hyphae independently of mating type. However, Hwp1 is selectively expressed on the wall of conjugation tubes formed by a/a cells, but not / cells, in the mating process. This was demonstrated in all possible crosses between four unrelated natural a/a strains and four unrelated / strains. In zygotes, Hwp1 is restricted to that portion of the wall of the conjugation bridge contributed by the a/a parent cell. Hwp1 staining further revealed that the first daughter bud that emerges from the conjugation bridge does so from the a/a-contributed portion. Hwp1 expression and localization during the mating process is, therefore, mating type specific, opaque phase specific, and -pheromone induced. These results indicate that the mating type-specific contributions to the conjugation bridge during the mating process in C. albicans are qualitatively and functionally distinct and that the a/a portion of the bridge, which selectively contains Hwp1, bears the first daughter cell in the mating process.

Corresponding author. E-mail address: david-soll{at}uiowa.edu.

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