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Vol. 15, Issue 1, 121-131, January 2004

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The Drosophila Kinesin-like Protein KLP67A Is Essential for Mitotic and Male Meiotic Spindle Assembly

Rita Gandhi ^*, Silvia Bonaccorsi , Diana Wentworth ^*, Stephen Doxsey , Maurizio Gatti, and Andrea Pereira 

^* Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01655; and Istituto Pasteur-Fondazione Cenci Bolognetti and Istituto di Biologia e Patologia Molecolari del Consiglio Nazionale delle Ricerche, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma "La Sapienza", P.le Aldo Moro 5, 00185 Rome, Italy

Submitted May 29, 2003; Revised August 20, 2003; Accepted August 28, 2003
Monitoring Editor: Ted Salmon

We have performed a mutational analysis together with RNA interference to determine the role of the kinesin-like protein KLP67A in Drosophila cell division. During both mitosis and male meiosis, Klp67A mutations cause an increase in MT length and disrupt discrete aspects of spindle assembly, as well as cytokinesis. Mutant cells exhibit greatly enlarged metaphase spindle as a result of excessive MT polymerization. The analysis of both living and fixed cells also shows perturbations in centrosome separation, chromosome segregation, and central spindle assembly. These data demonstrate that the MT plus end-directed motor KLP67A is essential for spindle assembly during mitosis and male meiosis and suggest that the regulation of MT plus-end polymerization is a key determinant of spindle architecture throughout cell division.

Online version of this article contains video material for some figures. Online version is available at www.molbiolcell.org.

Corresponding author. E-mail address: andrea.pereira{at}umassmed.edu.

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