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Originally published as MBC in Press, 10.1091/mbc.E03-10-0762 on January 12, 2004

Vol. 15, Issue 3, 1031-1043, March 2004

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Expression Profiling of Mammalian Male Meiosis and Gametogenesis Identifies Novel Candidate Genes for Roles in the Regulation of Fertility

Ulrich Schlecht * {dagger}, Philippe Demougin * {dagger}, Reinhold Koch * {dagger}, Leandro Hermida {dagger}, Christa Wiederkehr {dagger}, Patrick Descombes {ddagger}, Charles Pineau §, Bernard Jégou §, and Michael Primig {dagger} ||

{dagger} Biozentrum and Swiss Institute of Bioinformatics, 4056 Basel; Switzerland; {ddagger} Genomics Platform, National Center of Competence in Research Frontiers in Genetics, Université de Genève/CMU, 1211 Geneva 4, Switzerland; and § Group d'Etude de la Reproduction chez le Male-Institut National de la Santé et de la Recherche Médicale U. 435, Université de Rennes I, Campus de Beaulieu, 35042 Rennes cedex, Bretagne, France

Submitted October 24, 2003; Revised December 1, 2003; Accepted December 1, 2003
Monitoring Editor: Keith Yamamoto

We report a comprehensive large-scale expression profiling analysis of mammalian male germ cells undergoing mitotic growth, meiosis, and gametogenesis by using high-density oligonucleotide microarrays and highly enriched cell populations. Among 11,955 rat loci investigated, 1268 were identified as differentially transcribed in germ cells at subsequent developmental stages compared with total testis, somatic Sertoli cells as well as brain and skeletal muscle controls. The loci were organized into four expression clusters that correspond to somatic, mitotic, meiotic, and postmeiotic cell types. This work provides information about expression patterns of ~200 genes known to be important during male germ cell development. Approximately 40 of those are included in a group of 121 transcripts for which we report germ cell expression and lack of transcription in three somatic control cell types. Moreover, we demonstrate the testicular expression and transcriptional induction in mitotic, meiotic, and/or postmeiotic germ cells of 293 as yet uncharacterized transcripts, some of which are likely to encode factors involved in spermatogenesis and fertility. This group also contains potential germ cell-specific targets for innovative contraceptives. A graphical display of the data is conveniently accessible through the GermOnline database at http://www.germonline.org.

Article published online ahead of print. Mol. Biol. Cell 10.1091/mbc.E03–10–0762. Article and publication date are available at www.molbiolcell.org/cgi/doi/10.1091/mbc.E03-10-0762.

Online version of this article contains supplementary material. Online version is available at www.molbiolcell.org.

* These authors contributed equally to this study.

|| Corresponding author. E-mail address: michael.primig{at}unibas.ch.




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