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Vol. 15, Issue 6, 2742-2749, June 2004

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Cytoplasmic Dynein Nucleates Microtubules to Organize Them into Radial Arrays In Vivo

Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06032-1507

Submitted October 29, 2003; Revised March 15, 2004; Accepted March 17, 2004
Monitoring Editor: J. Richard McIntosh

Numerous evidence demonstrates that dynein is crucial for organization of microtubules (MTs) into radial arrays, but its exact function in this process is unclear. Here, we studied the role of cytoplasmic dynein in MT radial array formation in the absence of the centrosome. We found that dynein is a potent MT nucleator in vitro and that stimulation of dynein activity in cytoplasmic fragments of melanophores induces nucleation-dependent formation of MT radial array in the absence of the centrosome. This new property of dynein, in combination with its known role as an MT motor that is essential for MT array organization in the absence and presence of the centrosome, makes it a unique molecule whose activity is necessary and sufficient for the formation and maintenance of MT radial arrays in cells.

^* Corresponding author. E-mail address: rodionov{at}nso.uchc.edu.

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