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Vol. 16, Issue 3, 1026-1042, March 2005
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* Genome Institute of Singapore, Singapore 138672, Singapore;
Bioinformatics Institute, Singapore 138671, Singapore;
|| Institute for Infocomm Research, Singapore 119613, Singapore;
¶ Temasek Life Sciences Laboratory, 1 Research Link, NUS, Singapore 117604, Singapore;
# Department of Biological Sciences, National University of Singapore, Singapore 117597, Singapore; and
@ Department of Biochemistry, National University of Singapore, Singapore 117597, Singapore
Submitted April 10, 2004;
Accepted December 2, 2004
Monitoring Editor: John Pringle
Cell cycle progression is both regulated and accompanied by periodic changes in the expression levels of a large number of genes. To investigate cell cycle-regulated transcriptional programs in the fission yeast Schizosaccharomyces pombe, we developed a whole-genome oligonucleotide-based DNA microarray. Microarray analysis of both wild-type and cdc25 mutant cell cultures was performed to identify transcripts whose levels oscillated during the cell cycle. Using an unsupervised algorithm, we identified 747 genes that met the criteria for cell cycle-regulated expression. Peaks of gene expression were found to be distributed throughout the entire cell cycle. Furthermore, we found that four promoter motifs exhibited strong association with cell cycle phase-specific expression. Examination of the regulation of MCB motif-containing genes through the perturbation of DNA synthesis control/MCB-binding factor (DSC/MBF)-mediated transcription in arrested synchronous cdc10 mutant cell cultures revealed a subset of functional targets of the DSC/MBF transcription factor complex, as well as certain gene promoter requirements. Finally, we compared our data with those for the budding yeast Saccharomyces cerevisiae and found 
140 genes that are cell cycle regulated in both yeasts, suggesting that these genes may play an evolutionarily conserved role in regulation of cell cycle-specific processes. Our complete data sets are available at http://giscompute.gis.a-star.edu.sg/~gisljh/CDC.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
These authors contributed equally to this work.
Present address: Beijing Normal University, Beijing, China.
Address correspondence to: Jianhua Liu (liujh{at}gis.a-star.edu.sg).
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