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Originally published as MBC in Press, 10.1091/mbc.E04-04-0346 on December 22, 2004

Vol. 16, Issue 3, 1043-1055, March 2005

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Ablation of PRC1 by Small Interfering RNA Demonstrates that Cytokinetic Abscission Requires a Central Spindle Bundle in Mammalian Cells, whereas Completion of Furrowing Does Not{boxv}

Cristiana Mollinari * {dagger}, Jean-Philippe Kleman *, Yasmina Saoudi {ddagger}, Sandra A. Jablonski §, Julien Perard *, Tim J. Yen §, and Robert L. Margolis *

* Institut de Biologie Structurale J-P Ebel (Commissariat à l'Energie Atomique-Centre National de la Recherche Scientifique-Université Joseph Fournier), 38027 Grenoble Cedex 1, France; {ddagger} Laboratoire du Cytosquelette, Institut National de la Santé et de la Recherche Médicale U366, Département Réponse et Dynamique Cellulaire, Commissariat à l'Energie Atomique/Grenoble, 38054 Grenoble Cedex 9, France; and § Institute for Cancer Research, The Fox Chase Cancer Center, Philadelphia, PA 19111

Submitted April 28, 2004; Revised December 2, 2004; Accepted December 3, 2004
Monitoring Editor: Ted Salmon

The temporal and spatial regulation of cytokinesis requires an interaction between the anaphase mitotic spindle and the cell cortex. However, the relative roles of the spindle asters or the central spindle bundle are not clear in mammalian cells. The central spindle normally serves as a platform to localize key regulators of cell cleavage, including passenger proteins. Using time-lapse and immunofluorescence analysis, we have addressed the consequences of eliminating the central spindle by ablation of PRC1, a microtubule bundling protein that is critical to the formation of the central spindle. Without a central spindle, the asters guide the equatorial cortical accumulation of anillin and actin, and of the passenger proteins, which organize into a subcortical ring in anaphase. Furrowing goes to completion, but abscission to create two daughter cells fails. We conclude the central spindle bundle is required for abscission but not for furrowing in mammalian cells.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E04-04-0346) on December 22, 2004.

{boxv} The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

{dagger} Present address: Department of Cell Biology and Neurosciences, Italian National Institute of Health (Instituto Superiore di Sanitá), 00161 Rome, Italy.

Address correspondence to: Robert L. Margolis (margolis{at}ibs.fr).




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