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Vol. 15, Issue 12, 5431-5442, December 2004

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IC138 Is a WD-Repeat Dynein Intermediate Chain Required for Light Chain Assembly and Regulation of Flagellar Bending

Triscia W. Hendrickson ^*, Catherine A. Perrone , Paul Griffin ^*, Kristin Wuichet ^*, Joshua Mueller , Pinfen Yang , Mary E. Porter , and Winfield S. Sale ^* 

^* Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322; Department of Genetics/Cell and Developmental Biology, University of Minnesota, Minneapolis, MN 55455; and Department of Biology, Marquette University, Milwaukee, WI 53201

Submitted August 12, 2004; Revised September 20, 2004; Accepted September 22, 2004
Monitoring Editor: Thomas Pollard

Increased phosphorylation of dynein IC IC138 correlates with decreases in flagellar microtubule sliding and phototaxis defects. To test the hypothesis that regulation of IC138 phosphorylation controls flagellar bending, we cloned the IC138 gene. IC138 encodes a novel protein with a calculated mass of 111 kDa and is predicted to form seven WD-repeats at the C terminus. IC138 maps near the BOP5 locus, and bop5-1 contains a point mutation resulting in a truncated IC138 lacking the C terminus, including the seventh WD-repeat. bop5-1 cells display wild-type flagellar beat frequency but swim slower than wild-type cells, suggesting that bop5-1 is altered in its ability to control flagellar waveform. Swimming speed is rescued in bop5-1 transformants containing the wild-type IC138, confirming that BOP5 encodes IC138. With the exception of the roadblock-related light chain, LC7b, all the other known components of the I1 complex, including the truncated IC138, are assembled in bop5-1 axonemes. Thus, the bop5-1 motility phenotype reveals a role for IC138 and LC7b in the control of flagellar bending. IC138 is hyperphosphorylated in paralyzed flagellar mutants lacking radial spoke and central pair components, further indicating a role for the radial spokes and central pair apparatus in control of IC138 phosphorylation and regulation of flagellar waveform.

Corresponding author. E-mail address: win{at}cellbio.emory.edu.

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