QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 16, Issue 5, 2529-2543, May 2005

This Article Full Text Full Text (PDF) Supplemental Movies All Versions of this Article: E04-12-1090v1 16/5/2529    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by VerPlank, L. Articles by Li, R. Search for Related Content PubMed PubMed Citation Articles by VerPlank, L. Articles by Li, R.

Cell Cycle-regulated Trafficking of Chs2 Controls Actomyosin Ring Stability during Cytokinesis

Department of Cell Biology, Harvard Medical School, Boston, MA 02115

Submitted December 20, 2004; Revised February 7, 2005; Accepted March 2, 2005
Monitoring Editor: Anthony Bretscher

Cytokinesis requires the coordination of many cellular complexes, particularly those involved in the constriction and reconstruction of the plasma membrane in the cleavage furrow. We have investigated the regulation and function of vesicle transport and fusion during cytokinesis in budding yeast. By using time-lapse confocal microscopy, we show that post-Golgi vesicles, as well as the exocyst, a complex required for the tethering and fusion of these vesicles, localize to the bud neck at a precise time just before spindle disassembly and actomyosin ring contraction. Using mutants affecting cyclin degradation and the mitotic exit network, we found that targeted secretion, in contrast to contractile ring activation, requires cyclin degradation but not the mitotic exit network. Analysis of cells in late anaphase bearing exocyst and myosin V mutations show that both vesicle transport and fusion machineries are required for the completion of cytokinesis, but this is not due to a delay in mitotic exit or assembly of the contractile ring. Further investigation of the dynamics of contractile rings in exocyst mutants shows these cells may be able to initiate contraction but often fail to complete the contraction due to premature disassembly during the contraction phase. This phenotype led us to identify Chs2, a transmembrane protein targeted to the bud neck through the exocytic pathway, as necessary for actomyosin ring stability during contraction. Chs2, as the chitin synthase that produces the primary septum, thus couples the assembly of the extracellular matrix with the dynamics of the contractile ring during cytokinesis.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Rong Li (rli{at}hms.harvard.edu).

This article has been cited by other articles:

				D. J. Wright, E. Munro, M. Corbett, A. J. Bentley, N. J. Fullwood, S. Murray, and C. Price The Saccharomyces cerevisiae Actin Cytoskeletal Component Bsp1p Has an Auxiliary Role in Actomyosin Ring Function and in the Maintenance of Bud-Neck Structure Genetics, April 1, 2008; 178(4): 1903 - 1914. [Abstract] [Full Text] [PDF]

				S. Dischinger, A. Krapp, L. Xie, J. R. Paulson, and V. Simanis Chemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network J. Cell Sci., March 15, 2008; 121(6): 843 - 853. [Abstract] [Full Text] [PDF]

				N. Dyer, E. Rebollo, P. Dominguez, N. Elkhatib, P. Chavrier, L. Daviet, C. Gonzalez, and M. Gonzalez-Gaitan Spermatocyte cytokinesis requires rapid membrane addition mediated by ARF6 on central spindle recycling endosomes Development, December 15, 2007; 134(24): 4437 - 4447. [Abstract] [Full Text] [PDF]

				M. G. Giansanti, G. Belloni, and M. Gatti Rab11 Is Required for Membrane Trafficking and Actomyosin Ring Constriction in Meiotic Cytokinesis of Drosophila Males Mol. Biol. Cell, December 1, 2007; 18(12): 5034 - 5047. [Abstract] [Full Text] [PDF]

				A. A. Bicknell, A. Babour, C. M. Federovitch, and M. Niwa A novel role in cytokinesis reveals a housekeeping function for the unfolded protein response J. Cell Biol., July 30, 2007; 177(6): 1017 - 1027. [Abstract] [Full Text] [PDF]

				G. Zhang, R. Kashimshetty, K. E. Ng, H. B. Tan, and F. M. Yeong Exit from mitosis triggers Chs2p transport from the endoplasmic reticulum to mother-daughter neck via the secretory pathway in budding yeast J. Cell Biol., July 17, 2006; 174(2): 207 - 220. [Abstract] [Full Text] [PDF]

				R. Martin-Garcia and M.-H. Valdivieso The fission yeast Chs2 protein interacts with the type-II myosin Myo3p and is required for the integrity of the actomyosin ring J. Cell Sci., July 1, 2006; 119(13): 2768 - 2779. [Abstract] [Full Text] [PDF]

				M. K. Gatt and D. M. Glover The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis J. Cell Sci., June 1, 2006; 119(11): 2225 - 2235. [Abstract] [Full Text] [PDF]

				M. Lord, E. Laves, and T. D. Pollard Cytokinesis Depends on the Motor Domains of Myosin-II in Fission Yeast but Not in Budding Yeast Mol. Biol. Cell, November 1, 2005; 16(11): 5346 - 5355. [Abstract] [Full Text] [PDF]