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Vol. 16, Issue 11, 5258-5268, November 2005

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Pml39, a Novel Protein of the Nuclear Periphery Required for Nuclear Retention of Improper Messenger Ribonucleoparticles

Benoît Palancade ^*, Michela Zuccolo ^*, Sophie Loeillet , Alain Nicolas , and Valérie Doye ^*

^* Pores Nucléaires et Transport Nucléocytoplasmique, Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique; Recombinaison et Instabilité Génétique, Unité Mixte de Recherche 7147 Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, Institut Curie, Section de Recherche, 75248 Paris Cedex 05, France

Submitted June 13, 2005; Revised August 16, 2005; Accepted September 1, 2005
Monitoring Editor: Susan Wente

Using a genetic screen, we have identified a previously uncharacterized Saccharomyces cerevisiae open reading frame (renamed PML39) that displays a specific interaction with nucleoporins of the Nup84 complex. Localization of a Pml39-green fluorescent protein (GFP) fusion and two-hybrid studies revealed that Pml39 is mainly docked to a subset of nuclear pore complexes opposite to the nucleolus through interactions with Mlp1 and Mlp2. The absence of Pml39 leads to a specific leakage of unspliced mRNAs that is not enhanced upon MLP1 deletion. In addition, overexpression of PML39-GFP induces a specific trapping of mRNAs transcribed from an intron-containing reporter and of the heterogenous nuclear ribonucleoprotein Nab2 within discrete nuclear domains. In a nup60 mutant, Pml39 is mislocalized together with Mlp1 and Mlp2 in intranuclear foci that also recruit Nab2. Moreover, pml39 partially rescues the thermosensitive phenotypes of messenger ribonucleoparticles (mRNPs) assembly mutants, indicating that PML39 deletion also bypasses the requirement for normally assembled mRNPs. Together, these data indicate that Pml39 is an upstream effector of the Mlps, involved in the retention of improper mRNPs in the nucleus before their export.

Abbreviations used: FISH, in situ fluorescence hybridization; mRNP, messenger ribonucleoparticle; NPC, nuclear pore complex; SPB, spindle pole body.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Benoît Palancade (palancad{at}curie.fr).

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