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Originally published as MBC in Press, 10.1091/mbc.E05-07-0617 on October 5, 2005

Vol. 16, Issue 12, 5866-5879, December 2005

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The Aspergillus fumigatus StuA Protein Governs the Up-Regulation of a Discrete Transcriptional Program during the Acquisition of Developmental Competence{boxd}

Donald C. Sheppard *, Thomas Doedt {dagger}, Lisa Y. Chiang {dagger}, H. Stanley Kim {ddagger}, Dan Chen {ddagger}, William C. Nierman {ddagger} §, and Scott G. Filler {dagger}

* Departments of Microbiology and Immunology, McGill University, Montreal, Quebec H3A 2B4, Canada; {dagger} Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA 90502; {ddagger} The Institute for Genomic Research, Rockville, MD 20850; and § The George Washington University School of Medicine, Department of Biochemistry and Molecular Biology, Washington, DC 20037

Submitted July 12, 2005; Revised September 6, 2005; Accepted September 22, 2005
Monitoring Editor: Trisha Davis

Members of the Asm1p, Phd1p, Sok2p, Efg1p, and StuAp (APSES) family of fungal proteins regulate morphogenesis and virulence in ascomycetes. We cloned the Aspergillus fumigatus APSES gene encoding StuAp and demonstrated that stuA transcription is markedly up-regulated after the acquisition of developmental competence. A. fumigatus {Delta}stuA mutants were impaired in their ability to undergo asexual reproduction. Conidiophore morphology was markedly abnormal, and only small numbers of dysmorphic conidia were produced, which exhibited precocious germination. Whole genome transcriptional analysis during the onset of developmental competence was performed and identified a subset of developmentally regulated genes that were stuA dependent, including a cluster of putative secondary metabolite biosynthesis genes, genes encoding proteins implicated in the regulation of morphogenesis, and genes encoding allergens and other antigenic proteins. Additionally, hyphae of the {Delta}stuA mutant displayed reduced expression of the catalase gene CAT1 and were hypersusceptible to hydrogen peroxide.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E05-07-0617) on October 5, 2005.

{boxd} The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Donald C. Sheppard (donald.sheppard{at}mcgill.ca).




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