QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 16, Issue 12, 5710-5718, December 2005

This Article Full Text Full Text (PDF) Supplementary Material All Versions of this Article: E05-08-0706v1 16/12/5710    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fang, Y. Articles by Spector, D. L. Search for Related Content PubMed PubMed Citation Articles by Fang, Y. Articles by Spector, D. L.

Centromere Positioning and Dynamics in Living Arabidopsis Plants

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724

Submitted August 2, 2005; Revised September 13, 2005; Accepted September 15, 2005
Monitoring Editor: Joseph Gall

The organization and dynamics of the genome have been shown to influence gene expression in many organisms. Data from mammalian tissue culture cells have provided conflicting conclusions with regard to the extent to which chromatin organization is inherited from mother to daughter nuclei. To gain insight into chromatin organization and dynamics, we developed transgenic Arabidopsis lines in which centromeres were tagged with a green fluorescent protein fusion of the centromere-specific histone H3. Using four-dimensional (4-D) live cell imaging, we show that Arabidopsis centromeres are constrained at the nuclear periphery during interphase and that the organization of endoreduplicated sister centromeres is cell type dependent with predominant clustering in root epidermal cells and dispersion in leaf epidermal cells. 4-D tracking of the entire set of centromeres through mitosis, in growing root meristematic cells, demonstrated that global centromere position is not precisely transmitted from the mother cell to daughter cells. These results provide important insight into our understanding of chromatin organization among different cells of a living organism.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: David L. Spector (spector{at}cshl.edu).

This article has been cited by other articles:

				T. A. Dittmer, N. J. Stacey, K. Sugimoto-Shirasu, and E. J. Richards LITTLE NUCLEI Genes Affecting Nuclear Morphology in Arabidopsis thaliana PLANT CELL, September 1, 2007; 19(9): 2793 - 2803. [Abstract] [Full Text] [PDF]

				B. I. Lindhout, P. Fransz, F. Tessadori, T. Meckel, P. J.J. Hooykaas, and B. J. v. d. Zaal Live cell imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteins Nucleic Acids Res., August 17, 2007; (2007) gkm618v1. [Abstract] [Full Text] [PDF]

				I. U. Rafalska-Metcalf and S. M. Janicki Show and tell: visualizing gene expression in living cells J. Cell Sci., July 15, 2007; 120(14): 2301 - 2307. [Abstract] [Full Text] [PDF]

				I. Lermontova, V. Schubert, J. Fuchs, S. Klatte, J. Macas, and I. Schubert Loading of Arabidopsis Centromeric Histone CENH3 Occurs Mainly during G2 and Requires the Presence of the Histone Fold Domain PLANT CELL, October 1, 2006; 18(10): 2443 - 2451. [Abstract] [Full Text] [PDF]