![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 17, Issue 9, 3781-3792, September 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
*Program in Molecular Medicine and Biomedical Imaging Group, Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01605
Submitted February 14, 2006;
Revised May 9, 2006;
Accepted June 5, 2006
Monitoring Editor: Ben Margolis
Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT particles are composed of at least 17 polypeptides that are thought to contain binding sites for various cargos that need to be transported from their site of synthesis in the cell body to the site of assembly in the cilium. We show here that the IFT20 subunit of the particle is localized to the Golgi complex in addition to the basal body and cilia where all previous IFT particle proteins had been found. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. Strong knock down of IFT20 in mammalian cells blocks ciliary assembly but does not affect Golgi structure. Moderate knockdown does not block cilia assembly but reduces the amount of polycystin-2 that is localized to the cilia. This work suggests that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium.
The online version of this contains supplemental material at MBC Online (http://www.molbiolcell.org). This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-02-0133) on June 14, 2006.
Address correspondence to: Gregory J. Pazour (gregory.pazour{at}umassmed.edu)
Abbreviations used: HPA, Helix pomatia agglutinin; IFT, intraflagellar transport; RPE, retinal pigmented epithelium.
This article has been cited by other articles:
|
|
 |
|
  A. Kubo, A. Yuba-Kubo, S. Tsukita, S. Tsukita, and M. Amagai Sentan: A Novel Specific Component of the Apical Structure of Vertebrate Motile Cilia Mol. Biol. Cell, December 1, 2008; 19(12): 5338 - 5346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Hoeng, S. C. Dawson, S. A. House, M. S. Sagolla, J. K. Pham, J. J. Mancuso, J. Lowe, and W. Z. Cande High-Resolution Crystal Structure and In Vivo Function of a Kinesin-2 Homologue in Giardia intestinalis Mol. Biol. Cell, July 1, 2008; 19(7): 3124 - 3137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Krock and B. D. Perkins The intraflagellar transport protein IFT57 is required for cilia maintenance and regulates IFT-particle-kinesin-II dissociation in vertebrate photoreceptors J. Cell Sci., June 1, 2008; 121(11): 1907 - 1915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Cunningham and R. A. Kahn Cofactor D Functions as a Centrosomal Protein and Is Required for the Recruitment of the {gamma}-Tubulin Ring Complex at Centrosomes and Organization of the Mitotic Spindle J. Biol. Chem., March 14, 2008; 283(11): 7155 - 7165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Absalon, T. Blisnick, L. Kohl, G. Toutirais, G. Dore, D. Julkowska, A. Tavenet, and P. Bastin Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes Mol. Biol. Cell, March 1, 2008; 19(3): 929 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-C. Tsao and M. A. Gorovsky Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body J. Cell Sci., February 15, 2008; 121(4): 428 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Huang, D. R. Diener, A. Mitchell, G. J. Pazour, G. B. Witman, and J. L. Rosenbaum Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella J. Cell Biol., November 5, 2007; 179(3): 501 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Pathak, T. Obara, S. Mangos, Y. Liu, and I. A. Drummond The Zebrafish fleer Gene Encodes an Essential Regulator of Cilia Tubulin Polyglutamylation Mol. Biol. Cell, November 1, 2007; 18(11): 4353 - 4364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rompolas, L. B. Pedersen, R. S. Patel-King, and S. M. King Chlamydomonas FAP133 is a dynein intermediate chain associated with the retrograde intraflagellar transport motor J. Cell Sci., October 15, 2007; 120(20): 3653 - 3665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. D. Malone, C. T. Anderson, P. Tummala, R. Y. Kwon, T. R. Johnston, T. Stearns, and C. R. Jacobs Primary cilia mediate mechanosensing in bone cells by a calcium-independent mechanism PNAS, August 14, 2007; 104(33): 13325 - 13330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-i. Yoshimura, J. Egerer, E. Fuchs, A. K. Haas, and F. A. Barr Functional dissection of Rab GTPases involved in primary cilium formation J. Cell Biol., July 24, 2007; 178(3): 363 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hou, H. Qin, J. A. Follit, G. J. Pazour, J. L. Rosenbaum, and G. B. Witman Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella J. Cell Biol., February 26, 2007; 176(5): 653 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Robert, G. Margall-Ducos, J.-E. Guidotti, O. Bregerie, C. Celati, C. Brechot, and C. Desdouets The intraflagellar transport component IFT88/polaris is a centrosomal protein regulating G1-S transition in non-ciliated cells J. Cell Sci., February 15, 2007; 120(4): 628 - 637. [Abstract] [Full Text] [PDF]
|
|
