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Vol. 17, Issue 11, 4736-4747, November 2006

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A Survey of Essential Gene Function in the Yeast Cell Division Cycle

Lisa Yu^*,, Lourdes Peña Castillo^,, Sanie Mnaimneh^,, Timothy R. Hughes^,, and Grant W. Brown^*,

*Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8; Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada M5G 1L6; and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada M5S 3E1

Submitted May 1, 2006; Revised August 4, 2006; Accepted August 16, 2006
Monitoring Editor: Trisha Davis

Mutations impacting specific stages of cell growth and division have provided a foundation for dissecting mechanisms that underlie cell cycle progression. We have undertaken an objective examination of the yeast cell cycle through flow cytometric analysis of DNA content in TetO₇ promoter mutant strains representing 75% of all essential yeast genes. More than 65% of the strains displayed specific alterations in DNA content, suggesting that reduced function of an essential gene in most cases impairs progression through a specific stage of the cell cycle. Because of the large number of essential genes required for protein biosynthesis, G1 accumulation was the most common phenotype observed in our analysis. In contrast, relatively few mutants displayed S-phase delay, and most of these were defective in genes required for DNA replication or nucleotide metabolism. G2 accumulation appeared to arise from a variety of defects. In addition to providing a global view of the diversity of essential cellular processes that influence cell cycle progression, these data also provided predictions regarding the functions of individual genes: we identified four new genes involved in protein trafficking (NUS1, PHS1, PGA2, PGA3), and we found that CSE1 and SMC4 are important for DNA replication.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-04-0368) on August 30, 2006.

Address correspondence to: Grant W. Brown (grant.brown{at}utoronto.ca) or Timothy R. Hughes (t.hughes{at}utoronto)

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