Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E06-05-0437 on October 18, 2006

Vol. 17, Issue 12, 5356-5371, December 2006

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-05-0437v1
17/12/5356    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shimada, M.
Right arrow Articles by Kawahara, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shimada, M.
Right arrow Articles by Kawahara, H.

Proteasomal Ubiquitin Receptor RPN-10 Controls Sex Determination in Caenorhabditis elegansFormula

Masumi Shimada*, Kenji Kanematsu*, Keiji Tanaka{dagger}, Hideyoshi Yokosawa*, and Hiroyuki Kawahara*

*Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan; and {dagger}Department of Molecular Oncology, The Tokyo Metropolitan Institute of Medical Sciences, Tokyo 113-8613, Japan

Submitted May 22, 2006; Revised September 6, 2006; Accepted October 6, 2006
Monitoring Editor: Thomas Sommer

The ubiquitin-binding RPN-10 protein serves as a ubiquitin receptor that delivers client proteins to the 26S proteasome. Although ubiquitin recognition is an essential step for proteasomal destruction, deletion of the rpn-10 gene in yeast does not influence viability, indicating redundancy of the substrate delivery pathway. However, their specificity and biological relevance in higher eukaryotes is still enigmatic. We report herein that knockdown of the rpn-10 gene, but not any other proteasome subunit genes, sexually transforms hermaphrodites to females by eliminating hermaphrodite spermatogenesis in Caenorhabditis elegans. The feminization phenotype induced by deletion of the rpn-10 gene was rescued by knockdown of tra-2, one of sexual fate decision genes promoting female development, and its downstream target tra-1, indicating that the TRA-2–mediated sex determination pathway is crucial for the {Delta}rpn-10–induced sterile phenotype. Intriguingly, we found that co-knockdown of rpn-10 and functionally related ubiquitin ligase ufd-2 overcomes the germline-musculinizing effect of fem-3(gf). Furthermore, TRA-2 proteins accumulated in rpn-10-defective worms. Our results show that the RPN-10–mediated ubiquitin pathway is indispensable for control of the TRA-2–mediated sex-determining pathway.

Formula The online version of this contains supplemental material at MBC Online (http://www.molbiolcell.org).

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-05-0437) on October 18, 2006.

Address correspondence to: Hiroyuki Kawahara (kawahara{at}pharm.hokudai.ac.jp)




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
J. Hamazaki, K. Sasaki, H. Kawahara, S.-i. Hisanaga, K. Tanaka, and S. Murata
Rpn10-Mediated Degradation of Ubiquitinated Proteins Is Essential for Mouse Development
Mol. Cell. Biol., October 1, 2007; 27(19): 6629 - 6638.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.