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Originally published as MBC in Press, 10.1091/mbc.E06-09-0827 on November 15, 2006

Vol. 18, Issue 2, 362-368, February 2007

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Mitochondria-associated Yeast mRNAs and the Biogenesis of Molecular ComplexesFormula Formula

M. Garcia*, X. Darzacq*, T. Delaveau*, L. Jourdren{dagger}, R. H. Singer{ddagger}, and C. Jacq*,{dagger}

*Laboratoire de Génétique Moléculaire, Centre National de la Recherche Scientifique-Ecole Normale Supérieure and {dagger}Plateforme Transcriptome IFR36 Ecole Normale Supérieure, 75230 Paris Cedex 05, France; and {ddagger}Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461

Submitted September 15, 2006; Revised November 3, 2006; Accepted November 7, 2006
Monitoring Editor: Thomas Fox

The coherence of mitochondrial biogenesis relies on spatiotemporally coordinated associations of 800–1000 proteins mostly encoded in the nuclear genome. We report the development of new quantitative analyses to assess the role of local protein translation in the construction of molecular complexes. We used real-time PCR to determine the cellular location of 112 mRNAs involved in seven mitochondrial complexes. Five typical cases were examined by an improved FISH protocol. The proteins produced in the vicinity of mitochondria (MLR proteins) were, almost exclusively, of prokaryotic origin and are key elements of the core construction of the molecular complexes; the accessory proteins were translated on free cytoplasmic polysomes. These two classes of proteins correspond, at least as far as intermembrane space (IMS) proteins are concerned, to two different import pathways. Import of MLR proteins involves both TOM and TIM23 complexes whereas non-MLR proteins only interact with the TOM complex. Site-specific translation loci, both outside and inside mitochondria, may coordinate the construction of molecular complexes composed of both nuclearly and mitochondrially encoded subunits.

Formula Formula The online version of this contains supplemental material at MBC Online (http://www.molbiolcell.org).

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-09-0827) on November 15, 2006.

Address correspondence to: Claude Jacq (jacq{at}biologie.ens.fr)

Abbreviations used: MLR, mitochondria-localized mRNA.




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Yeast Mitochondrial Initiator tRNA Is Methylated at Guanosine 37 by the Trm5-encoded tRNA (Guanine-N1-)-methyltransferase
J. Biol. Chem., September 21, 2007; 282(38): 27744 - 27753.
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