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Vol. 18, Issue 9, 3375-3387, September 2007

This Article Full Text Full Text (PDF) Supplemental Material A correction has been published All Versions of this Article: E07-01-0070v1 18/9/3375    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lian, S. Articles by Chan, E. K.L. Search for Related Content PubMed PubMed Citation Articles by Lian, S. Articles by Chan, E. K.L.

Small Interfering RNA-mediated Silencing Induces Target-dependent Assembly of GW/P Bodies

Shangli Lian^*, Marvin J. Fritzler, Joseph Katz, Takashi Hamazaki, Naohiro Terada, Minoru Satoh^,||, and Edward K.L. Chan^*

Departments of *Oral Biology, Oral Medicine, and Pathology, Immunology, and Laboratory Medicine, and ^||Division of Rheumatology and Clinical Immunology, Department of Medicine, University of Florida, Gainesville, FL 32610; and Department of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada T2N 1N4

Submitted January 26, 2007; Revised June 18, 2007; Accepted June 19, 2007
Monitoring Editor: A. Gregory Matera

Gene silencing using small interfering RNA (siRNA) is a valuable laboratory tool and a promising approach to therapeutics for a variety of human diseases. Recently, RNA interference (RNAi) has been linked to cytoplasmic GW bodies (GWB). However, the correlation between RNAi and the formation of GWB, also known as mammalian processing bodies, remains unclear. In this report, we show that transfection of functional siRNA induced larger and greater numbers of GWB. This siRNA-induced increase of GWB depended on the endogenous expression of the target mRNA. Knockdown of GW182 or Ago2 demonstrated that the siRNA-induced increase of GWB required these two proteins and correlated with RNAi. Furthermore, knockdown of rck/p54 or LSm1 did not prevent the reassembly of GWB that were induced by and correlated with siRNA-mediated RNA silencing. We propose that RNAi is a key regulatory mechanism for the assembly of GWB, and in some cases, GWB may serve as markers for RNAi in mammalian cells.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Edward K.L. Chan (echan{at}ufl.edu).

Abbreviations used: GWB, GW bodies; IIF, indirect immunofluorescence; miRNA, microRNA; P bodies, processing bodies; RISC, RNA-induced silencing complex; SG, stress granules.