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Vol. 18, Issue 12, 4698-4710, December 2007

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Late Endosomal Traffic of the Epidermal Growth Factor Receptor Ensures Spatial and Temporal Fidelity of Mitogen-activated Protein Kinase Signaling

N. Taub^*, D. Teis, H. L. Ebner, M. W. Hess, and L. A. Huber^*

*Division of Cell Biology, Biocenter, Innsbruck Medical University, A-6020 Innsbruck, Austria; Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY 14853; and Division of Histology and Embryology, Innsbruck Medical University, A-6020 Innsbruck, Austria

Submitted February 2, 2007; Revised August 27, 2007; Accepted September 7, 2007
Monitoring Editor: Jean Gruenberg

Mitogen-activated protein kinase (MAPK) signaling is regulated by assembling distinct scaffold complexes at the plasma membrane and on endosomes. Thus, spatial resolution might be critical to determine signaling specificity. Therefore, we investigated whether epidermal growth factor receptor (EGFR) traffic through the endosomal system provides spatial information for MAPK signaling. To mislocalize late endosomes to the cell periphery we used the dynein subunit p50 dynamitin. The peripheral translocation of late endosomes resulted in a prolonged EGFR activation on late endosomes and a slow down in EGFR degradation. Continuous EGFR signaling from late endosomes caused sustained extracellular signal-regulated kinase and p38 signaling and resulted in hyperactivation of nuclear targets, such as Elk-1. In contrast, clustering late endosomes in the perinuclear region by expression of dominant active Rab7 delayed the entry of the EGFR into late endosomes, which caused a delay in EGFR degradation and a sustained MAPK signaling. Surprisingly, the activation of nuclear targets was reduced. Thus, we conclude that appropriate trafficking of the activated EGFR through endosomes controls the spatial and temporal regulation of MAPK signaling.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Lukas A. Huber (lukas.a.huber{at}i-med.ac.at).

Abbreviations used: EEA1, early endosomal antigen 1; HRP, horseradish peroxidase; LAMP1, lysosomal-associated membrane protein 1; LBPA, lysobiphosphatic acid; MVB, multivesicular body; p-ERK, phospho-extracellular signal-regulated kinase; p-p38, phospho-p38.

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