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Originally published as MBC in Press, 10.1091/mbc.E07-05-0519 on November 7, 2007

Vol. 19, Issue 1, 284-296, January 2008

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Large-Scale Analysis of Yeast Filamentous Growth by Systematic Gene Disruption and Overexpression

Rui Jin, Craig J. Dobry, Phillip J. McCown, and Anuj Kumar

Department of Molecular, Cellular, and Developmental Biology and Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2216

Submitted June 1, 2007; Revised September 10, 2007; Accepted October 29, 2007
Monitoring Editor: Charles Boone

Under certain conditions of nutrient stress, the budding yeast Saccharomyces cerevisiae initiates a striking developmental transition to a filamentous form of growth, resembling developmental transitions required for virulence in closely related pathogenic fungi. In yeast, filamentous growth involves known mitogen-activated protein kinase and protein kinase A signaling modules, but the full scope of this extensive filamentous response has not been delineated. Accordingly, we have undertaken the first systematic gene disruption and overexpression analysis of yeast filamentous growth. Standard laboratory strains of yeast are nonfilamentous; thus, we constructed a unique set of reagents in the filamentous {Sigma}1278b strain, encompassing 3627 integrated transposon insertion alleles and 2043 overexpression constructs. Collectively, we analyzed 4528 yeast genes with these reagents and identified 487 genes conferring mutant filamentous phenotypes upon transposon insertion and/or gene overexpression. Using a fluorescent protein reporter integrated at the MUC1 locus, we further assayed each filamentous growth mutant for aberrant protein levels of the key flocculence factor Muc1p. Our results indicate a variety of genes and pathways affecting filamentous growth. In total, this filamentous growth gene set represents a wealth of yeast biology, highlighting 84 genes of uncharacterized function and an underappreciated role for the mitochondrial retrograde signaling pathway as an inhibitor of filamentous growth.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E07-05-0519) on November 7, 2007.

Address correspondence to: Anuj Kumar (anujk{at}umich.edu)




This article has been cited by other articles:


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N. Bharucha, J. Ma, C. J. Dobry, S. K. Lawson, Z. Yang, and A. Kumar
Analysis of the Yeast Kinome Reveals a Network of Regulated Protein Localization during Filamentous Growth
Mol. Biol. Cell, July 1, 2008; 19(7): 2708 - 2717.
[Abstract] [Full Text] [PDF]


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