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Vol. 19, Issue 4, 1646-1662, April 2008

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Sister Kinetochore Recapture in Fission Yeast Occurs by Two Distinct Mechanisms, Both Requiring Dam1 and Klp2

Laboratoire de Biologie Cellulaire et Moléculaire du Controle de la Prolifération (LBCMCP), Centre National de la Recherche Scientifique, Université de Toulouse, 31062 Toulouse, France

Submitted September 17, 2007; Revised January 17, 2008; Accepted January 30, 2008
Monitoring Editor: David Drubin

In eukaryotic cells, proper formation of the spindle is necessary for successful cell division. We have studied chromosome recapture in the fission yeast Schizosaccharomyces pombe. We show by live cell analysis that lost kinetochores interact laterally with intranuclear microtubules (INMs) and that both microtubule depolymerization (end-on pulling) and minus-end–directed movement (microtubule sliding) contribute to chromosome retrieval to the spindle pole body (SPB). We find that the minus-end–directed motor Klp2 colocalizes with the kinetochore during its transport to the SPB and contributes to the effectiveness of retrieval by affecting both end-on pulling and lateral sliding. Furthermore, we provide in vivo evidence that Dam1, a component of the DASH complex, also colocalizes with the kinetochore during its transport and is essential for its retrieval by either of these mechanisms. Finally, we find that the position of the unattached kinetochore correlates with the size and orientation of the INMs, suggesting that chromosome recapture may not be a random process.

* These authors contributed equally to this work.

Address correspondence to: Sylvie Tournier (tournier{at}cict.fr)

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