Biogenesis of N-Cadherin-dependent Cell-Cell Contacts in Living Fibroblasts Is a Microtubule-dependent Kinesin-driven Mechanism182
- J. Richard McIntosh, Monitoring Editor
- Submitted July 2, 2001.
- Revised October 4, 2001.
- Accepted October 22, 2001.
Abstract
Cadherin-mediated cell-cell adhesion is a dynamic process that is regulated during embryonic development, cell migration, and differentiation. Different cadherins are expressed in specific tissues consistent with their roles in cell type recognition. In this study, we examine the formation of N-cadherin–dependent cell-cell contacts in fibroblasts and myoblasts. In contrast to E-cadherin, both endogenous and ectopically expressed N-cadherin shuttles between an intracellular and a plasma membrane pool. Initial formation of N-cadherin–dependent cell-cell contacts results from the recruitment of the intracellular pool of N-cadherin to the plasma membrane. N-cadherin also localizes to the Golgi apparatus and both secretory and endocytotic vesicles. We demonstrate that the intracellular pool of N-cadherin is tightly associated with the microtubule (MT) network and that junction formation requires MTs. In addition, localization of N-cadherin to the cortex is dependent on an intact F-actin cytoskeleton. We show that N-cadherin transport requires the MT network as well as the activity of the MT-associated motor kinesin. In conclusion, we propose that N-cadherin distribution is a regulated process promoted by cell-cell contact formation, which controls the biogenesis and turnover of the junctions through the MT network.
Footnotes
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↵182 Online version of this article contains video material for Figures 4, 5, 8, and 9. Online version available at www.molbiolcell.org.
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↵* Corresponding author. E-mail address:gauthier{at}crbm.cnrs-mop.fr.
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Article published online ahead of print. Mol. Biol. Cell 10.1091/mbc.01–07-0337. Article and publication date are atwww.molbiolcell.org/cgi/doi/10.1091/mbc.01–07-0337.
Abbreviations used:
- GFP
- green fluorescent protein
- Ncad/GFP
- N-cadherin/GFP
- MT
- microtubule
- Nz
- nocodazole
- IF
- intermediate filament
