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Cover  A classic paper by Mark Mooseker and Lew Tilney (1975, "Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells." J. Cell Biol. 67, 725-742) described the organization of the actin filaments in the brush border of the intestinal epithelial cells. The cover reproduces an electron micrograph of a thin section parallel to the microvilli showing the organization of the actin filament bundles. Mooseker and Tilney used decoration with myosin subfragment-1 to establish that all of the actin filaments have the same polarity with their barbed ends at the tips of the microvilli. They also discovered lateral connections between the actin filament bundles and the surrounding plasma membrane (later shown to be brush border myosin-I) and connections between the filament bundles in the terminal web (later shown to be myosin-II). This highly ordered assembly of actin filaments became a model for thinking about how actin filaments are organized in the cortex of many other cells. Reproduced from The Journal of Cell Biology, 1975, 67, 725-742, by copyright permission of The Rockefeller University Press.Thomas Pollard