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The image depicts two cells in the salivary glands of the dipteran Chironomus tentans after in situ hybridization with fluorescent probes against Balbiani ring messenger RNA (BR mRNA) (red) and 28S ribosomal RNA (rRNA) (green). The polytene chromosomes have been stained by DAPI (blue). The left cell has been mock-injected and exhibits a Balbiani ring (red), the transcription site of BR mRNA, and a nucleolus (green), the transcription site of rRNA. The right cell has been injected by an antibody against Nup153, a protein of the nuclear pore complex. BR mRNA and rRNA have accumulated in the nucleoplasm. The transport is blocked at the nuclear pore, which is further investigated at the ultrastructural level in an article by Soop et al. on p. 5610 of this issue. (Image: Bertil Daneholt and Teresa Soop, Karolinska Institutet)