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CFTR protein expression in well-differentiated human bronchial epithelial cultures. Well-differentiated cultures derived from human bronchial epithelial tissues were immunostained with CFTR and tubulin antibodies and analyzed on a Leica SP2 laser confocal microscope with a PlanApo 63x NA1.2 lens and three independent laser lines (364, 488, and 568 nm). The image represents an overlay of the DIC (grayscale), CFTR (redorange), cilia (tubulin, green), and nuclei (DAPI, blue) confocal planes and depicts an epithelial cell sheet that contains a group of ciliated cells surrounding a goblet cell (bottle-shaped cell with no cilia). CFTR is expressed only at the apical membrane of ciliated cells, but not goblet cells. Magnification x190.