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Trichomes of an Arabidopsis thaliana plant expressing the 14-3-3-φ/GFP transgene fluoresce green on a background of red chlorophyll autofluorescence. The subcellular localization pattern of 14-3-3-φ/GFP within each trichome is characteristic of the φ isoform fusion protein and is distinct from the localization pattern displayed by several other 14-3-3-isoform-φ/GFP fusions. The localization pattern suggests that 14-3-3-φ/GFP interacts with protein clients associated with the plasma membrane and nucleus and with cytoskeletal components. The characterization of 14-3-3-φ/GFP and other Arabidopsis 14-3-3 isoforms tagged with GFP are described in Paul et al. (Mol. Biol. Cell [2005] 16, 1735-1743; published online before print as 10.1091/ mbc.E04-09-0839.) (Image, Anna- Lisa Paul and Robert Ferl, University of Florida)