Arp2/3 complex inhibition radically alters lamellipodial actin architecture, suspended cell shape, and the cell spreading process
Supplemental Material
combinedsupmats.pdf (1 MB)
1Address correspondence to: John Henson (E-mail Address: [email protected]).
Department of Biology, Dickinson College, Carlisle, PA 17013
Marine Biological Laboratory, Woods Hole, MA 02543
Department of Biology, Dickinson College, Carlisle, PA 17013
Department of Biology, Dickinson College, Carlisle, PA 17013
Department of Biology, Dickinson College, Carlisle, PA 17013
Department of Biology, Dickinson College, Carlisle, PA 17013
Department of Biology, Dickinson College, Carlisle, PA 17013
Department of Biology, Dickinson College, Carlisle, PA 17013
Marine Biological Laboratory, Woods Hole, MA 02543
Marine Biological Laboratory, Woods Hole, MA 02543
Department of Biology, New Mexico State University, Las Cruces, NM 88003
combinedsupmats.pdf (1 MB)
© 2015 Henson et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
We extend grateful thanks to Tanya Svitkina and Maria Shutova (University of Pennsylvania, Philadelphia, PA) for their expert assistance with the generation of platinum replicas of coelomocyte cytoskeletons, to the members of the Dickinson College Spring 2013 Biology 560 research course (Brandon Goldson, Caroline Jordan, Jake Kleiner, Christine Neville, Rebecca Patterson, and Eileen Shen) for conducting helpful preliminary experiments, to Callen Wallace and Simon Watkins (University of Pittsburgh, Pittsburgh, PA) for assistance with superresolution 3D SIM light microscopy, to Amitabh Verma and Shalin Mehta (Marine Biological Laboratory) for software engineering help with LC-PolScope acquisition, and to Louie Kerr (Marine Biological Laboratory) for excellent assistance with TEM imaging. This research was supported by National Science Foundation STEP grant 0856704 to Dickinson College, student/faculty summer research grants from the Dickinson College Research and Development Committee, Laura and Arthur Colwin Summer Research Fellowships from the Marine Biological Laboratory to J.H.H. and C.B.S., National Institutes of Health Grant EB002583 to R.O., and National Science Foundation collaborative research grants to J.H.H. (MCB-1412688) and C.B.S. (MCB-1412734).