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*Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536; and

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Garland L. Crawford

*Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536; and

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Zubair A. Karim

*Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536; and

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Chunxia Zhao

*Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536; and

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Wangsun Choi

*Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536; and

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Cheng-Chun Wang

Membrane Biology Laboratory, Institute of Molecular and Cellular Biology, Proteos, Singapore 138673, Singapore

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Wanjin Hong

Membrane Biology Laboratory, Institute of Molecular and Cellular Biology, Proteos, Singapore 138673, Singapore

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, and

Sidney W. Whiteheart

*Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536; and

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Sean Munro, Monitoring Editor

Published Online:25 Oct 2006https://doi.org/10.1091/mbc.e06-09-0785

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History

Submitted: 5 September 2006

Revised: 5 October 2006

Accepted: 16 October 2006

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ACKNOWLEDGMENTS

The authors thank Dr. Thomas Südhof and Dr. Jeffery Pessin for the synaptobrevin/VAMP-2+/− and cellubrevin/VAMP-3−/− mice, respectively. We thank Dr. Theirry Galli for his gifts of reagents. We are indebted to the Central Kentucky Blood Center and to members of the Whiteheart Laboratory, especially Dr. Elena Matveeva and Dr. Todd Schraw, for critical reading of the manuscript. We thank Dr. Bill Dean for assistance in measuring intraplatelet calcium and Dr. Bruce Maley and Ms. Mary Gail Engels for their assistance with the electron microscopy. There is no conflict of interest to declare for any of the authors. This work was supported by National Heart, Lung, and Blood Institute (National Institutes of Health, Bethesda, MD) (to S.W.W.) and by the Ohio Valley Affiliate of the American Heart Association (to G.L.C., Q.R., and W.C.).

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Endobrevin/VAMP-8 Is the Primary v-SNARE for the Platelet Release Reaction

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